Composite

Part:BBa_K4160009:Design

Designed by: Femi Hesen, Wouter Langers, Floor van Boxtel   Group: iGEM22_TU-Eindhoven   (2022-10-09)


GEMS receptor construct containing PR3 as affinity domain


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 733
    Illegal XbaI site found at 2339
    Illegal PstI site found at 191
    Illegal PstI site found at 1027
    Illegal PstI site found at 2022
    Illegal PstI site found at 2183
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 733
    Illegal NheI site found at 1396
    Illegal PstI site found at 191
    Illegal PstI site found at 1027
    Illegal PstI site found at 2022
    Illegal PstI site found at 2183
    Illegal NotI site found at 2326
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 733
    Illegal BglII site found at 1500
    Illegal BglII site found at 1686
    Illegal BglII site found at 1950
    Illegal BamHI site found at 64
    Illegal XhoI site found at 910
    Illegal XhoI site found at 2333
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 733
    Illegal XbaI site found at 2339
    Illegal PstI site found at 191
    Illegal PstI site found at 1027
    Illegal PstI site found at 2022
    Illegal PstI site found at 2183
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 733
    Illegal XbaI site found at 2339
    Illegal PstI site found at 191
    Illegal PstI site found at 1027
    Illegal PstI site found at 2022
    Illegal PstI site found at 2183
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

-


Source

This composite part combines BBa_K4160000, BBa_K4160001, BBa_K4160002, BBa_K4160004, and BBa_K2217015

References